Journal: Communications Biology

Article Title: Alteration in tyrosine phosphorylation of cardiac proteome and EGFR pathway contribute to hypertrophic cardiomyopathy

doi: 10.1038/s42003-022-04021-4

Figure Lengend Snippet: a Myofilament from Ntg ( n = 3) and ErbB2 ( n = 3) transgenic mice hearts were freshly isolated on ice-cold buffers containing Proteinase and Phosphatase Inhibitors Cocktails (Roche). b All material was resuspended in TEAB, then reduced and alkylated. Tryptic peptides were desalted and labeled with 6-plex isobaric tandem mass tags (TMT). c The digested and labeled peptides were pooled and desalted with C 18 SEP-PAK. The enrichment for phosphotyrosine was performed with PTMScan Phospho-Tyrosine Rabbit mAb ( P -Tyr-1000) kit (Cell Signaling Technology). The eluted peptide samples were desalted using C18 STAGE tips d Easy-nanoLC 1200 nanoflow liquid chromatography system coupled to Orbitrap Fusion Lumos Tribrid.

Article Snippet: The digested and labeled peptides were pooled and desalted with C 18 SEP-PAK (Waters), followed by pTyrsoine enrichment using PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) kit (Cell Signaling Technology), see Fig. .

Techniques: Transgenic Assay, Isolation, Labeling, Liquid Chromatography

Journal: Neuropharmacology

Article Title: Phosphoproteomics implicates glutamatergic and dopaminergic signalling in the antidepressant-like properties of the iron chelator deferiprone.

doi: 10.1016/j.neuropharm.2024.109837

Figure Lengend Snippet: Fig. 5 Effect of acute deferiprone treatment on pTH-Ser40, TH protein levels and the ratio of

Article Snippet: Blots were then incubated overnight with the primary antibodies against pTH-Ser40 (anti-rabbit; Cell Signalling Technology; CAT#2791) diluted in TBST containing 3% (w/v) BSA at 4°C.

Techniques: